Optimization of Nutritive Factors in Culture Media for Growth and Tropane Alkaloid Production from Anisodus acutangulus Hairy Roots

Article history: Received on: 05/11/2012 Revised on: 29/11/2012 Accepted on: 18/12/2012 Available online: 28/02/2013 Anisodus acutangulus hairy roots were grown in N6 medium which was optimal for growth and alkaloid production. The cell biomass and alkaloid yield reached 2.7 g l and 3.9 mg l (dry weight) respectively in sucrose medium, higher than those obtained in other carbon source media. 90 mM nitrogen (NH4/NO3 = 4:1) gave the highest cell yield (4.5 g l) and the maximum alkaloid production (9.9 mg l). The cell yield (4.1 g l) of hairy roots grown at pH 6.5 was 2 times higher than that at pH 4.5. However, the maximum alkaloid production (7.2 mg l) was yielded at pH 4.5.